Super Script Ⅱ阴性逆转录酶

HM0064

Super script Ⅱ阴性逆转录酶

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BR,生化研究

Superscript II Reverse Transcriptase

-20℃

来源：Purified from E. coli expressing the pol gene of M-MLV (1,2), mutagenized to reduce the RNase H activity
浓度：200u/ul

活力定义：One unit of Superscript® II is the amount of enzyme required to incorporate 1 nmole of eoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as template•primer (3).

Unit Reaction Conditions：50 mM Tris-HC1 (pH 8.3), 40 mM KC1, 6 mM MgC1 2 , 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18 , 0.1 mg/m1 BSA, and enzyme in 50 µl for 10 min. at 37℃

Performance and Quality Testing：SDS-PAGE purity；endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product

Enzyme Function：RNA dependent DNA Polymerase

Final Product Size：12.3 kb or less

Number of Reactions：10 Reactions

Optimal Reaction Temperature：42℃

Sensitivity：Medium

Template：ssRNA；ssDNA；RNA-DNA Hybrid

产品描述：Superscript® II Reverse Transcriptase (RT) is an improved version of Superscript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations rather than a deletion in the RNase H active center. Like Superscript® RT, Superscript® II RT has reduced RNase H activity. Unlike Superscript® RT, however, the selective mutations within the RNase H domain maintain full polymerase activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives Superscript® II RT superior performance characteristics, including：Greater first-strand cDNA yields；More full-length cDNA synthesis；Full activity at 42℃

阴性逆转录酶	Superscript II Reverse Transcriptase  BR	10000U	¥8200
阴性逆转录酶	Superscript II Reverse Transcriptase  BR	2000U	¥2200