Background: Rab-4 belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20, RUFY1, SGSM1, SGSM2 and SGSM3. It participates in protein transport and in vesicular traffic.
Immunogen: Recombinant full length protein of active Rab4A.
Applications: IP, IHC
Recommended Dilutions: 1 μg for 1~2 mg total cellular proteins for IP
1:50~100 dilution for IHC
Concentration: 1 mg/ml
Host: mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Rab4 antibody recognizes active Rab4 from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Immunoprecipitation/Western blot:
Rab4A activation assay. Purified GST-tagged Rab4A proteins (Cat. #10130) were immunoprecipitated with the anti-active Rab4 monoclonal antibody (Cat. No. 26921) after treated with GDP (lane 1) or GTPγ S(lane 2), and was blotted with anti-Rab4A polyclonal antibody (Cat. No. 21067). Input control is shown in the bottom panel.
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