Background: Rab3 is a member of the Rab-family GTPases and is specifically targeted to synaptic vesicles.
Immunogen: Recombinant full length protein of active Rab3A
Applications: IP, IHC, IF
Recommended Dilutions:
1 µg for ~2 mg total cellular proteins for IP
1:100 dilution for IHC, IF
Concentration: 1 mg/ml
Host: mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Rab3 antibody recognizes active Rab3 proteins of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Immunoprecipitation/Western blot:
Rab3 activation assay. Purified full-length Rab3A proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab3 monoclonal antibody (Cat. No. 26920). Immunoblot was with an anti-Rab3A polyclonal antibody (Cat. No. 21041).
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