Background: The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions.
Immunogen: Recombinant full length protein of active Rab35
Applications: IP, IHC, IF
Recommended Dilutions:
1 µg for ~2 mg total cellular proteins for IP
1:100 dilution for IHC, IF
Concentration: 1 mg/ml
Host: mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Rab35 antibody recognizes active Rab35 proteins of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Immunoprecipitation/Western blot:
Rab35 activation assay. Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab35 monoclonal antibody (Cat. No. 26922). Immunoblot was with an anti-Rab35 polyclonal antibody (Cat. No. 21078).
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