Background: Rab7 belongs to the Rab family of small GTPases. Rab7 is located in the late endosome, lysosome, cytoplasmic vesicle, phagosome and melanosome. It is involved in the late endocytic transport and contributes to the maturation of phagosomes.
Immunogen: Recombinant full length protein of active Rab7
Applications: IP, IHC, IF
Recommended Dilutions:
1 µg for ~2 mg total cellular proteins for IP
1:100 dilution for IHC, IF
Concentration: 1 mg/ml
Host: mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Rab7 antibody recognizes active Rab7 proteins of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Immunoprecipitation/Western blot:
Rab7 activation assay. Purified full-length Rab7 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab7 monoclonal antibody (Cat. No. 26923). Immunoblot was with an anti-Rab7 polyclonal antibody (Cat. No. 21069).
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