Background: Rab5 is a member of Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.
Immunogen: Recombinant full length protein of active Gao
Applications: IP, IHC
Recommended Dilutions: 1 µg for ~2 mg total cellular proteins for IP
1:100 dilution for IHC
Concentration: 1 mg/ml
Host: mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG1
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Rab5 antibody recognizes active Rab5 proteins of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Immunoprecipitation/Western blot:
Rab5 activation assay. Purified full-length Rab5A proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab5 monoclonal antibody (Cat. No. 26911). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21151).
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