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Catalog Number: 26234
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Gene Symbol: PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
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Description: Anti-PIK3CA (E542K) Mouse Monoclonal Antibody
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Background: Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
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Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of E542K, human origin.
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Applications: ELISA, WB
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Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:2000
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Concentration: 1 mg/ml
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Host: Mouse
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Format: Liquid
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Clonality: Monoclonal
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Isotype:
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Purity: Purified from ascites
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Storage buffer:
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Preservative: no
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Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
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Species Reactivity: Recognizes E542K mutant, but not wild-type PIK3CA of vertebrates.
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Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
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Western blot:
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Western blot analysis of recombinant PIK3CA (E542K) and wildtype proteins. Purified His-tagged PIK3CA (E542K) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-PIK3CA (E542K) monoclonal antibody (Cat. #26234).
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