Background: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas.
Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: recognizes R172K mutant, but not wild-type IDH2 of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of recombinant IDH2 (R172K) and wildtype proteins. Purified His-tagged IDH2 (R172K) (lane 2) and wildtype protein (lane 1) were blotted with anti-IDH2 (R172K) monoclonal antibody (Cat. #26163).
Immunofluorescence:
Immunofluorescence of cells expressing IDH2 proteins with anti-IDH2 (R172K) antibody. HEK293T cells were transfected with pCDNA3-GFP- IDH2 (R172K) plasmid , pCDNA3-GFP- IDH2 (WT) plasmid, pCDNA3-GFP- IDH2 (R172H) plasmid , pCDNA3-GFP- IDH2 (R172W) plasmid, pCDNA3-GFP- IDH2 (R172G) plasmid,pCDNA3-GFP- IDH2 (R172M) plasmid or pCDNA3-GFP- IDH2 (R172S) plasmid, then fixed and stained with anti- IDH2 (R172K) monoclonal antibody (Cat. #26163)
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