Catalog Number: 26164
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Gene Symbol: IDH2 (R172W)
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Description: Anti-IDH2 (R172W) Mouse Monoclonal Antibody
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Background: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172W IDH2 mutations do exist in diffusely infiltrative gliomas.
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Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172W, human origin.
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Applications: ELISA, WB, IHC
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Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100
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Concentration: 1 mg/ml
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Host: Mouse
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Format: Liquid
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Clonality: Monoclonal
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Isotype:
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Purity: Purified from ascites
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Storage buffer:
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Preservative: no
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Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
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Species Reactivity: recognizes IDH1(R172W) of vertebrates.
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Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
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Western blot:
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