Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαz represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαz proteins are being explored.
Immunogen: Recombinant full length protein of active Gαz
Applications: IP, IHC
Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC
Concentration: 1 mg/ml
Host: mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Gαz antibody recognizes active Gαz of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Immunoprecipitation/Western blot:
Gαz activation assay. Purified Gαz proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαz monoclonal antibody (Cat. No. 26908). Immunoblot was with an anti-Gαz polyclonal antibody.
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