2. 在ELISA中,进行各项实验条件的选择是很重要的,其中包括:
(1) 固相载体的选择:许多物质可作为固相载体,如聚氯乙烯、聚苯乙烯、聚丙酰胺和纤维素等。其形式可以是凹孔平板、试管、珠粒等。当前常用的是40孔聚苯乙烯凹孔板。不管何种载体,在使用前均可进行筛选:用等量抗原包被,在同一实验条件下进行反应,观察其显色反应是否均一性,据此判明其吸附性能是否良好。
(2) 包被抗体(或抗原)的选择:将抗体(或抗原)吸附在固相载体表面时,要求纯度要好,吸附时一般要求PH在9.0~9.6之间。吸附温度,时间及其蛋白量也有一定影响,一般多采用4℃18~24小时。蛋白质包被的最适浓度需进行滴定:即用不同的蛋白质浓度(0.1、1.0和10μg/ml等)进行包被后,在其它试验条件相同时,观察阳性标本的OD值。选择OD值最大而蛋白量最少的浓度。对于多数蛋白质来说通常为1~10μg/ml。
(3)酶标记抗体工作浓度的选择:首先用直接ELISA法进行初步效价的滴定(见酶标记抗体部份)。然后再固定其它条件或采取“方阵法”(包被物、待检样品的参考品及酶标记抗体分别为不同的稀释度)在正式实验系统里准确地滴定其工作浓度。
(4) 酶的底物及供氢体的选择:对供氢体的选择要求是价廉、安全、有明显地显色反应,而本身无色。有些供氢体(如OPD等)有潜在的致癌作用,应注意防护。有条件者应使用不致癌、灵敏度高的供氢体,如TMB和ABTS是当前较为满意的供氢体。底物作用一段时间后,应加入强酸或强碱以终止反应。通常底物作用时间,以10-30分钟为宜。底物使用液必须新鲜配制,尤其是H2O2在临用前加入。
供应产品信息
兔抗人C1GALT1特异性伴侣1多克隆抗体
Rabbit anti-human basigin (Ok blood group) polyclonal Antibody
兔抗人basigin(OK血型)多克隆抗体
Rabbit anti-human bone marrow stromal cell antigen 2 polyclonal Antibody
兔抗人骨髓基质干细胞抗原2多克隆抗体
Rabbit anti-human BTB (POZ) domain containing 1 polyclonal Antibody
兔抗人BTB(POZ)含1多克隆抗体域
Rabbit anti-human BTB (POZ) domain containing 10 polyclonal Antibody
兔抗人BTB(POZ)含10多克隆抗体域
Rabbit anti-human beta cellulin,BTC polyclonal Antibody
兔抗人β动物纤维素,BTC多克隆抗体
Rabbit anti-human biotinidase polyclonal Antibody
兔抗人生物素酶多克隆抗体
Rabbit anti-human B-cell translocation gene 1, anti-proliferative polyclonal Antibody
兔抗人B细胞易位基因1、抗proliferative多克隆抗体
Rabbit anti-human B-cell translocation gene 4 polyclonal Antibody
兔抗人B细胞易位基因4多克隆抗体
Rabbit anti-human Bruton agammaglobulinemia tyrosine kinase polyclonal Antibody
兔抗人Bruton丙种酪氨酸kinase多克隆抗体
Rabbit anti-human butyrophilin, subfamily 2, member A1 polyclonal Antibody
兔抗人butyrophilin,亚科2,A1多克隆抗体成员
Rabbit anti-human butyrophilin, subfamily 2, member A2 polyclonal Antibody
兔抗人butyrophilin,亚科2,A2多克隆抗体成员
Rabbit anti-human butyrophilin, subfamily 3, member A3 polyclonal Antibody
兔抗人butyrophilin,亚科3,A3多克隆抗体成员
Rabbit anti-human budding uninhibited by benzimidazoles 1 homolog beta (yeast) polyclonal Antibody
兔抗人buddinguninhibitedbybenzimidazoles1homologbeta(yeast)多克隆抗体
Rabbit anti-human budding uninhibited by benzimidazoles 3 homolog (yeast) polyclonal Antibody
兔抗人buddinguninhibitedbybenzimidazoles3homolog(yeast)多克隆抗体
Rabbit anti-human BUD31 homolog (S. cerevisiae) polyclonal Antibody
兔抗人BUD31同源(酵母)多克隆抗体
Rabbit anti-human blood vessel epicardial substance polyclonal Antibody
兔抗人blood血管心外膜substance多克隆抗体
Rabbit anti-human brix domain containing 1 polyclonal Antibody
兔抗人brixdomaincontaining1多克隆抗体
Rabbit anti-human brix domain containing 2 polyclonal Antibody
兔抗人brixdomaincontaining2多克隆抗体
Rabbit anti-human basic leucine zipper and W2 domains 1 polyclonal Antibody
兔抗人basic亮氨酸拉链蛋白和W2域1多克隆抗体
Rabbit anti-human chromosome 10 open reading frame 119 polyclonal Antibody
兔抗人染色体10开放阅读框架119多克隆抗体
Rabbit anti-human chromosome 11 open reading frame 49 polyclonal Antibody
兔抗人染色体11开放阅读框架49多克隆抗体
Rabbit anti-human chromosome 11 open reading frame 51 polyclonal Antibody
兔抗人染色体11开放阅读框架51多克隆抗体
Rabbit anti-human chromosome 11 open reading frame 67 polyclonal Antibody
兔抗人染色体11开放阅读框架67多克隆抗体
Rabbit anti-human chromosome 11 open reading frame 70 polyclonal Antibody
兔抗人染色体11开放阅读框架70多克隆抗体
Rabbit anti-human chromosome 11 open reading frame 73 polyclonal Antibody
兔抗人染色体11开放阅读框架73多克隆抗体
Rabbit anti-human chromosome 12 open reading frame 11 polyclonal Antibody
兔抗人染色体12开放阅读框架11多克隆抗体
Rabbit anti-human chromosome 13 open reading frame 18 polyclonal Antibody
兔抗人染色体13开放阅读框架18多克隆抗体
Rabbit anti-human chromosome 14 open reading frame 133 polyclonal Antibody
兔抗人染色体14开放阅读框架133多克隆抗体
Rabbit anti-human chromosome 14 open reading frame 166 polyclonal Antibody
兔抗人染色体14开放阅读框架166多克隆抗体
Rabbit anti-human chromosome 14 open reading frame 94 polyclonal Antibody
兔抗人染色体14开放阅读框架94多克隆抗体
Rabbit anti-human chromosome 16 open reading frame 14 polyclonal Antibody
兔抗人染色体16开放阅读框架14多克隆抗体
Rabbit anti-human chromosome 16 open reading frame 70 polyclonal Antibody
兔抗人染色体16开放阅读框架70多克隆抗体
Rabbit anti-human chromosome 17 open reading frame 28 polyclonal Antibody
兔抗人染色体17开放阅读框架28多克隆抗体
Rabbit anti-human chromosome 18 open reading frame 55 polyclonal Antibody
兔抗人染色体18开放阅读框架55多克隆抗体
Rabbit anti-human chromosome 18 open reading frame 8 polyclonal Antibody
兔抗人染色体18开放阅读框架8多克隆抗体
Rabbit anti-human chromosome 19 open reading frame 10 polyclonal Antibody
兔抗人染色体19开放阅读框架10多克隆抗体
Rabbit anti-human chromosome 19 open reading frame 43 polyclonal Antibody
兔抗人染色体19开放阅读框架43多克隆抗体
Rabbit anti-human C1D nuclear receptor co-repressor polyclonal Antibody
兔抗人C1D核受体辅抑制物多克隆抗体
Rabbit anti-human C1GALT1-specific chaperone 1 polyclonal Antibody
兔抗人C1GALT1特异性伴侣1多克隆抗体