氨[快速]检测试剂盒 , Ammonia (Rapid) Assay Kit , 货号：K-AMIAR
中文品名：氨[快速]检测试剂盒
品名：Ammonia (Rapid) Assay Kit
规格： 96次(手工) / 960次 (微孔板) / 960次(自动分析仪)
应用：氨[快速]检测试剂盒，用于包括葡萄汁和葡萄酒以及其它食品、饮料样本中氨含量的快速检测。 辅助因子更强的稳定性，溶解后的辅助因子在4°C下可保存超过1年。适合手工、微孔板和自动分析仪分析。
原理：
食品、饮料和其它食材中氨的紫外检测方法
检测方法：分光光度法 @340 nm
反应时间：~ 5分钟
检测限： 0.07 mg/L
应用案例：
葡萄汁、葡萄酒、水果汁、软饮料、奶制品（例如牛奶）、营养食品、酱油、蛋及蛋制品、奶酪、肉、加工肉、海鲜、烘焙产品、化肥、医药、烟草、化妆品、水、凯氏定氮、纸张（和纸板）、水和其他材料 （例如：生物培养、样品等）
方法识别：
基于此原理的检测方法被MEBAK接受认可。
试剂盒组成：
|
Bottle 1:
|
缓冲液（36 ml，pH 8），添加α-酮戊二酸和叠氮化钠（0.02% w/v）作为防腐剂
|
|
|
4°C下稳定超过 2年
|
|
Bottle 2:
|
NADPH
|
|
|
-20°C稳定超过5年
|
|
Bottle 3:
|
谷氨酸脱氢酶(2.2 mL）
|
|
|
4°C稳定超过2年
|
|
Bottle 4:
|
氨标准溶液(5 mL, 0.04 mg/mL) 溶于0.02% (w/v)叠氮钠
|
|
|
4°C稳定超过2年
|
优点：
使用谷氨酸脱氢酶，反应异常迅速
提供稳定的酶悬浮液 价格非常具有竞争力
所有试剂制备后可以稳定保存超过2年
官网提供Mega-Calc™ 软件工具用于一站式原始数据处理
包含标准品
辅助因子更强的稳定性
适合手工、微孔板和自动分析仪分析
参考文献：
Grape and wine analysis: Oenologists to exploit advanced test kits. Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.
Megazyme “advanced” wine test kits general characteristics and validation. Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.
Combined intracellular nitrate and NIT2 effects on storage carbohydrate metabolism in Chlamydomonas. Remacle, C., Eppe, G., Coosemans, N., Fernandez, E. & Vigeolas, H. (2014). Journal of Experimental Botany, 65(1), 23-33.
A
mutation in GDP-mannose pyrophosphorylase causes conditional
hypersensitivity to ammonium, resulting in Arabidopsis root growth
inhibition, altered ammonium metabolism, and hormone homeostasis. Barth, C., Gouzd, Z. A., Steele, H. P. & Imperio, R. M. (2010). Journal of Experimental Botany, 61(2), 379-394.
Proteomic
phenotyping of Novosphingobium nitrogenifigens reveals a robust
capacity for simultaneous nitrogen fixation, polyhydroxyalkanoate
production, and resistance to reactive oxygen species. Smit, A. M.,
Strabala, T. J., Peng, L., Rawson, P., Lloyd-Jones, G. & Jordan, T.
W. (2012). Applied and Environmental Microbiology, 78(14), 4802-4815.
No
effects of gluten in patients with self-reported non-celiac gluten
sensitivity after dietary reduction of fermentable, poorly absorbed,
short-chain carbohydrates. Biesiekierski, J. R., Peters, S. L.,
Newnham, E. D., Rosella, O., Muir, J. G. & Gibson, P. R. (2013).
Gastroenterology, 145(2), 320-328.
The
interaction between high ammonia diet and bile duct ligation in
developing rats: assessment by spatial memory and asymmetric
dimethylarginine. Huang, L. T., Chen, C. C., Sheen, J. M., Chen, Y.
J., Hsieh, C. S. & Tain, Y. L. (2010). International Journal of
Developmental Neuroscience, 28(2), 169-174.
Dietary
protein excess during neonatal life alters colonic Microbiota and
mucosal response to inflammatory mediators later in life in female pigs. Boudry, G., Jamin, A., Chatelais, L., Gras-Le Guen, C., Michel, C.
& Le Huërou-Luron, I. (2013). The Journal of Nutrition, 143(8),
1225-1232.
Effects
of enzymatic modification of wheat protein on the formation of
pyrazines and other volatile components in the Maillard reaction. Lee, S. E., Chung, H. & Kim, Y. S. (2012). Food Chemistry, 131(4), 1248-1254.
使用手册：
相关产品：
L-Arginine/Urea/Ammonia Assay Kit
Primary Amino Nitrogen Assay Kit (PANOPA)
Urea/Ammonia Assay Kit (Rapid)
L-Asparagine/L-Glutamine/Ammonia Assay Kit (Rapid)
L-Glutamine/Ammonia Assay Kit (Rapid)
D-Fructose/D-Glucose Assay Kit
L-Malic Acid Assay Kit (Manual Format)
Total Sulfite Assay Kit
通过认证 [诚信档案]
![氨[快速]检测试剂盒 （K-AMIAR）检测原理](http://www.seebio.cn/UploadFiles/FCK/2017-03/2017030226Z8NFH0PR.png "氨[快速]检测试剂盒 （K-AMIAR）检测原理")
![α-萘乙酸[NAA]【科研、组培用】](http://www.cnbio.net/file/upload/202409/04/17171473211147.jpg.middle.jpg)
![6-苄氨基嘌呤[6-BA]【科研、组织培养用】](http://www.cnbio.net/file/upload/202409/04/17140735211147.jpg.middle.jpg)