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西宝生物科技(上海)股份有限公司
地址:上海市张江高科技园区毕昇路299弄11号502室 邮编:201204
电话:021-50272978
西宝市场部 手机:13917439331 QQ:2852385819
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Anti Iba1, Rabbit
019-19741(For Immunocytochemistry) & 016-20001 (for Western Blotting)
Iba1: ionized calcium binding adapter molecule 1 小胶质细胞/巨噬细胞特异性蛋白抗体
钙离子是已知的所有细胞,包括中枢神经系统(CNS)细胞中最重要的信号调解人之一。钙离子通过与各种钙结合蛋白结合发挥信号活动,其中许多钙结合蛋白被划分成一个大的蛋白家族,EF手性蛋白家族。Iba1是一个17 kDa的EF手性蛋白,在巨噬细胞和小胶质细胞中表达,并在这些细胞的活化过程中表达升高。
本抗体以合成的的Iba1羧基末端序列为抗原,该序列是人,大鼠和小鼠Iba1蛋白质保守序列。本抗体(免疫组化用)特异性与小胶质细胞/巨噬细胞反应,免疫双重染色与单克隆抗体结合,可与GFAP单克隆抗体((胶质纤维酸性蛋白,Glial Fibrillary Acidic Protein)联合使用对脑组织和细胞培养物进行双重染色。
特点
S特异性检测小胶质细胞与/巨噬细胞,不与神经细胞与星形胶质细胞发生交叉反应。可与人,大鼠,小鼠的Iba1反应
货号 |
品名 |
包装 |
用途 |
工作浓度 |
019-19741 |
Anti Iba1 polyclonal antibody, Rabbit, for Immunocytochemistry |
50ug(100ul) |
IH |
1-2ug/ml |
016-20001 |
Anti Iba1 polyclonal antibody, Rabbit, for Western Blotting |
50ug(100ul) |
WB |
0.5-1ug/ml |
石蜡切片应用方法:
1. 固定
2. 用0.01M PBS缓冲液清洗二次
3. 使用含有0.3% 过氧化氢的甲醇孵育30分钟
4. 用0.01M PBS缓冲液清洗三次
5. 使用封闭液(含有1.5%的山羊血清和1%BSA的0.001M PBS缓冲液)室温孵育2小时
6. 使用含有Anti Iba1 polyclonal antibody的封闭液4℃孵育过夜
7. 用0.01M PBS缓冲液清洗三次
8. 使用含有生物素标记的抗兔IgG(1:200)的封闭液室温孵育1小时
9. 用0.01M PBS缓冲液清洗三次
10. 使用Elite ABC试剂(含有试剂A1:50,试剂B1:50的0.01M PBS溶液)
11. 用0.01M PBS缓冲液清洗三次
12. 过氧化物酶溶液(含有0.01% 过氧化氢和0.05% DAB的0.05M Tris溶液)
西宝市场部 手机:13917439331 QQ:2852385819
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电话:021-50272978 13917439331 QQ:2852385819
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电话:021-50272978 13917439331 QQ:2852385819