密理博Amicon Ultra-0.5 mL超滤离心管

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品牌：Millipore
所属分类：实验耗材 » 过滤,层析杂交用耗材 » 超滤
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密理博Amicon Ultra-0.5 mL超滤离心管

Millipore Amicon Ultra 0.5ml超滤离心管

Amicon Ultra-0.5 mL Centrifugal Filters for DNA and Protein Purification and Concentration

With their vertical membrane design, Amicon Ultra 0.5 mL filters deliver great performance and lower spin times.

**订货信息**
货号	描述	NMWL (KDa)	包装
UFC500308	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-3 membrane	3	8
UFC500324	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-3 membrane	3	24
UFC500396	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-3 membrane	3	96
UFC5003BK	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-3 membrane	3	500
UFC501008	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-10 membrane	10	8
UFC501024	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-10 membrane	10	24
UFC501096	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-10 membrane	10	96
UFC5010BK	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-10 membrane	10	500
UFC503008	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-30 membrane	30	8
UFC503024	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-30 membrane	30	24
UFC503096	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-30 membrane	30	96
UFC5030BK	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-30 membrane	30	500
UFC505008	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 membrane	50	8
UFC505024	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 membrane	50	24
UFC505096	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 membrane	50	96
UFC5050BK	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 membrane	50	500
UFC510008	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-100 membrane	100	8
UFC510024	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-100 membrane	100	24
UFC510096	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-100 membrane	100	96
UFC5100BK	Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-100 membrane	100	500

概述

Concentrates 500 µL down to 15 µL
Concentration factor 25 x to 30 x
High sample recovery (greater than 90% of dilute starting solution)
Fast processing time - 10 to 30 minutes
Consistent recoveries thanks to the reverse spin capability
Reverse spin capability provides consistent recoveries

amicon0.5-spin

Applications

Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
Purification of macromolecular components found in tissue culture extracts or cell lysates
Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC
Desalting, buffer exchange and protein dialysis
Size exclusion and protein fractionation

Material Supplied
The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample.

Performance – DNA Concentration
The Amicon Ultra-0.5 device provides the best balance between recovery and spin time for double-stranded DNA for base pairs ranging from 137 to 1159. To achieve maximum PCR product recovery and primer removal with primers greater than 20 bases, one or two additional spins with Tris-EDTA (TE) buffer are recommended.

Typical Recovery of Nucleotides from Amicon Ultra-0.5 30K Devices
PCR Product (base pairs)	PCR Primer (bases)	PCR Recovery (%)	PCR Primer Removal (%)	TE Washes (number)
137	10	≥95	≥90	0
	20	≥90	≥85	1
	48	≥90	≥75	2
301	10	≥90	≥90	0
	20	≥85	≥90	1
	48	≥90	≥80	2
648	10	≥95	≥90	0
	20	≥90	≥90	1
	48	≥95	≥90	2
1159	10	≥90	≥90	0
	20	≥90	≥95	1
	48	≥95	≥95	2
Spin conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume, 20-30 µL final volume, 10 minute spin, n=12.

amicon05-new

Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume. Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=8.

Typical Concentrate Volume/Concentration Factor vs. Spin Time

Concentrate Volume / Concentration Factor
	3K device		10K device		30K device		50K device		100K device
Spin time (min)	Conc. Volume (µL)	Conc. Factor (x)	Conc. Volume (µL)	Conc. Factor (x)	Conc. Volume (µL)	Conc. Factor (x)	Conc. Volume (µL)	Conc. Factor (x)	Conc. Volume (µL)	Conc. Factor (x)
5	215	2	66	7	42	12	28	18	58	9
10	114	4	35	14	23	22	20	25	19	26
15	80	6	22	22	19	27	17	30	15	33
20	62	8	20	24	17	30	15	33	13	36
30	48	10	15	31	15	32	15	36	11	41
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume. Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=12. Shaded volumes were used for the calculation of protein recovery in the Typical Concentrate Recovery table.

Typical Retention of Protein Markers

Marker/Concentration	Molecular Weight	Device NMWL	% Retention	Spin Time (min)
α-Chymotrypsinogen (1 mg/mL)	25,000	3K	> 95	30
Cytochrome c (0.25 mg/mL)	12,400		> 95	30
Vitamin B-12 (0.2 mg/mL)	1,350		< 42	30
α-Chymotrypsinogen (1 mg/mL)	25,000	10K	> 95	15
Cytochrome c (0.25 mg/mL)	12,400		> 95	15
Vitamin B-12 (0.2 mg/mL)	1,350		< 42	15
BSA (1 mg/mL)	67,000	30K	> 95	10
Ovalbumin (1 mg/mL)	45,000		> 95	10
Cytrochorme c (0.25 mg/mL)	12,400		< 35	10
BSA (1 mg/mL)	67,000	50K	> 95	10
Ovalbumin (1 mg/mL)	45,000		~ 40	10
Cytrochorme c (0.25 mg/mL)	12,400		< 20	10
Thyroglobulin (0.5 mg/mL)	677,000	100K	> 95	10
IgG (1 mg/mL)	156,000		> 95	10
Ovalbumin (1 mg/mL)	45,000		< 30	10
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume, n=12.

Typical Concentrate Recovery

Marker/Concentration	Molecular Weight	Device NMWL	Spin Time (min)	Concentrate Volume (µL)	Concentration Factor (x)	Concentrate Recovery (%)
Cytochrome c (0.25 mg/mL)	12,400	3K	30	48	10	98
Cytochrome c (0.25 mg/mL)	12,400	10K	15	22	22	93
BSA (1 mg/mL)	67,000	30K	10	23	22	97
BSA (1 mg/mL)	67,000	50K	10	20	25	92